Relationship of gas vesicles volume and gvp gene transcript level in temperature influence

Authors

  • Zhang Yongsheng * , Li Haiying , Wan Yu

Abstract

Algae blooms usually appear in marine shallows, lakes, and reservoirs. Gas vesicle is regarded as a
major buoyancy provider during algae cells floating. However, the relationship between gas
vesicles volume and gvp gene transcription level at different temperatures is still very unclear.
Furthermore, it was difficult to determine the temperature threshold of gas vesicle volume change
significantly because of the environment complexity and the ecological research methods
limitations. In the research, gas vesicles volume was detected by capillary compression tubes. Three
gvpA copies and a gvpC gene transcription level were also detected by real-time qPCR at a
temperature from 9°C to 33°C. The temperature threshold leading to gas vesicle volume change
significantly was determined by reducing temperature interval. The results were as follow: Gas
vesicles volume gradually increased from 9°C to 22°C, increased quickly from 22°C to 28°C, and
decreased slightly from 28°C to 33°C. The changing trend of gvpA2 transcription level was
consistent with that of gas vesicles volume. Gas vesicle volume changed significantly from 22°C to
23°C. We considered that it is feasible to analyze the change of gas vesicles volume through
analyzing that of gvp gene transcription level.

Published

2020-10-16

Issue

Section

Articles