Genetic sequence of Cryptosporidium parvum association with enteric bacterial infections

Abstract

In this study, (100) subjects were included, (55) of them were diarrheal patients with irritable bowel
syndrome (IBS) who were referred to Baghdad Teaching Hospital during the period from 1st
September 2019 to 1st February 2020, and (45) of them were apparently healthy subjects as a control
group. The distribution of C. parvum infection was significantly highest 23(23%) among the age group
(31-50) years (P<0.01). Flatulence was the most common feature 70(70.0%) with a highly significant
difference followed by abdominal pain 59(59.0%), while the less (22.0%) common feature was
vomiting (15%) with non significant difference. A number of enteric bacteria were found to be
associated with cryptosporidiosis in these cases. For the detection of rRNA gene of C parvum by using
the RFLP PCR with ladder 1000 bp which was found in 600bp. The genetic change which was found
in 10 position TGT>TAT, ATA>ACA, TCA>TAA, TTA>AGA, AAT>GAT, GAT>GGT,
CAT>CGT, AAT>GAT,TAA>TGA, AAA>AAG, AAT>GAT, AAT>GAT, ATA>GCA,
AAT>GAT, TTG>TCG, ATT>ACT, AAT>GAT, TAT>TCG, AAT>GAT, ATA>ATG, TAT, TCG
of 18S rRNA gene sequences explained the resistance of C. parvum against the conditions within the
host's body and the drugs.